ISBN: 9780470095027
Integrated Genomics: A Discovery-Based Laboratory Course introduces the excitement of discovery to the basic molecular biology laboratory. Utilizing up-to-date molecular biology protocols… Más…
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ISBN: 9780470095027
Integrated Genomics: A Discovery-Based Laboratory Course introduces the excitement of discovery to the basic molecular biology laboratory. Utilizing up-to-date molecular biology protocols… Más…
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ISBN: 9780470095027
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ISBN: 9780470095027
Integrated Genomics: A Discovery-Based Laboratory Course introduces the excitement of discovery to the basic molecular biology laboratory. Utilizing up-to-date molecular biology protocols… Más…
ISBN: 9780470095027
Integrated Genomics: A Discovery-Based Laboratory Course introduces the excitement of discovery to the basic molecular biology laboratory. Utilizing up-to-date molecular biology protocols… Más…
2006
ISBN: 9780470095027
Pasta blanda
[ED: Kartoniert / Broschiert], [PU: Wiley & Sons], Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Über den AutorGuy A. Caldwell, Ph.D… Más…
ISBN: 9780470095027
*Integrated Genomics* - A Discovery-Based Laboratory Course. 1. Auflage / Taschenbuch für 107.99 € / Aus dem Bereich: Bücher, Wissenschaft, Biologie Medien > Bücher nein Buch (kartoniert)… Más…
2006, ISBN: 9780470095027
Buch, Softcover, [PU: John Wiley & Sons Inc], John Wiley & Sons Inc, 2006
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Detalles del libro - Integrated Genomics
EAN (ISBN-13): 9780470095027
ISBN (ISBN-10): 0470095024
Tapa dura
Tapa blanda
Año de publicación: 2006
Editorial: John Wiley & Sons
225 Páginas
Peso: 0,576 kg
Idioma: eng/Englisch
Libro en la base de datos desde 2008-06-12T23:22:41+02:00 (Madrid)
Página de detalles modificada por última vez el 2023-04-13T12:36:07+02:00 (Madrid)
ISBN/EAN: 9780470095027
ISBN - escritura alterna:
0-470-09502-4, 978-0-470-09502-7
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Autor del libro: shelli, williams, guy, kim, john caldwell
Título del libro: integrated genomics discovery based laboratory course
Datos del la editorial
Autor: Guy Caldwell; Shelli Williams; Kim Caldwell
Título: Integrated Genomics - A Discovery-Based Laboratory Course
Editorial: Wiley; John Wiley & Sons
248 Páginas
Año de publicación: 2006-07-28
Peso: 0,719 kg
Idioma: Inglés
79,90 € (DE)
Not available (reason unspecified)
216mm x 279mm x 15mm
BC; Hardcover, Softcover / Biologie/Genetik, Gentechnik; Genetik (nicht-medizinisch); Biowissenschaften; Genetics; Genetik; Genomics; Life Sciences; Genetik; BB
Preface. Author biographies. Acknowledgments. List of figures. 1 Introduction to basic laboratory genetics. 1.1 Transferring and handling C. elegans. 1.2 Introduction to laboratory genetics. 2 Gene expression analysis using transgenic animals. 2.1 Transgenic gene expression analysis in C. elegans: lacZ staining. 2.2 Transgenic gene expression analysis in C. elegans: GFP analysis. 3 Creation and testing of transgenic yeast for use in protein-protein interaction screening. 3.1 Small-scale transformation of S. cerevisiae. 3.2 Transformation of S. cerevisiae to test for non-specific interaction. 3.3 Assaying for protein-protein interaction by reporter gene expression. 4 Yeast two-hybrid screening. 4.1 Protein-protein interaction screening of a C. elegans cDNA library. 4.2 Assaying for protein-protein interaction by reporter gene expression. 5 Isolation and identification of interacting proteins. 5.1 Preparation of electrocompetent E. coli. 5.2 Isolation of DNA from yeast and electroporation of E. coli. 5.3 Small-scale isolation of plasmid DNA from E. coli: the mini-prep. 5.4 Sequencing of two-hybrid library plasmid DNA vectors. 6 Using bioinformatics in modern science. 6.1 DNA sequence chromatogram. 6.2 BLASTing your sequence. 6.3 Evaluating sequence results and choosing an RNAi target. 6.4 Bioinformatics practice questions. 7 Generation of an RNAi vector. 7.1 Small-scale isolation of genomic DNA from C. elegans. 7.2 PCR amplification of target gene sequence from C. elegans genomic DNA. 7.3 Preparations for cloning to generate RNAi vector. 7.3.1 Agarose gel electrophoresis. 7.3.2 Removal of dNTPs from PCR reaction. 7.3.3 Restriction enzyme digestion of PCR product and C. elegans RNAi vector. 7.4 Gel purification of DNA and ligation of vector and PCR-amplified DNA. 7.4.1 Preparative agarose gel electrophoresis. 7.4.2 Gel purification of DNA from agarose gel. 7.4.3 Ligation of vector and PCR-amplified DNA. 7.5 Transformation of ligation reactions. 7.6 PCR screening of transformation colonies. 7.7 Small-scale isolation of plasmid DNA from E. coli: the mini-prep. 7.8 Verifying successful ligation by restriction digestion. 8 RNA-mediated interference by bacterial feeding. 8.1 Preparation of RNAi-feeding bacteria for transformation. 8.2 Media preparation for RNAi feeding. 8.3 Transformation of RNAi-feeding strain HT115(DE3). 8.4 RNA interference by bacterial feeding of C. elegans. 8.5 Analyzing effects of dsRNAi. 8.5.1 Assaying for sterility (Ste) or embryonic lethality (Emb). 8.5.2 Assaying for growth effect. 8.5.3 Assaying for morphological effects. 8.5.4 Assaying for general neuromuscular effects. 8.5.5 Assaying for specific neuronal effects. 8.5.6 Assaying for dauer formation. Appendix I Recombinational cloning. AI.1 Isolation of genomic DNA from C. elegans. AI.2 PCR amplification of target gene sequence from C. elegans genomic DNA. AI.3 Agarose gel electrophoresis and clean-up of PCR reaction. AI.4 Entry vector cloning. AI.5 Small-scale isolation of plasmid DNA from E. coli: the mini-prep. AI.6 Destination vector cloning. AI.7 Small-scale isolation of plasmid DNA from E. coli: the mini-prep. Appendix II Recipes and media preparation. Solution recipes. Media preparation. Appendix III Sterile techniques and worm protocols. Sterile techniques. Worm protocols. Appendix IV Mutant C. elegans phenotypes. Appendix V Vector maps. Subject index.Más, otros libros, que pueden ser muy parecidos a este:
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